An efficient protocol for peanut (Arachis hypogaea L.) transformation mediated by Agrobacterium rhizogenes
نویسندگان
چکیده
The Agrobacterium-mediated transformation of hairy roots creates a rapid and simple means to introduce and express foreign genes in plant cells that are capable of synthesizing specific natural products. We describe a method for the production of transgenic hairy root cultures of peanut (Arachis hypogaea L.) using Agrobacterium rhizogenes R1000, a strain with the binary vector pBI121. We evaluated the stable transformation of kanamycin-resistant hairy roots by determining the integration of the NPTП gene into the plant genome, the histochemical localization of GUS activity, the presence of GUS mRNA, and the level of GUS enzyme activity. Transgenic root culture of A. hypogaea is a simple, reliable and well-defined model system to investigate the molecular and metabolic regulation of resveratrol biosynthesis, and to evaluate the metabolic engineering potential of peanut.
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